validation of an analytical method for determination of benzo[a]pyrene bread using quechers method by gc-ms

Authors

samira eslamizad department of pharmacology and toxicology, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran

yazdanpanah hassan yazdanpanah department of pharmacology and toxicology, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran

katayoun javidnia medicinal and natural products chemistry research centre, shiraz university of medical sciences, shiraz, iran

ramezan sadeghi department of pharmacology and toxicology, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran

abstract

a fast and simple modified quechers (quick, easy, cheap, rugged and safe) extraction method based on spiked calibration curves and direct sample introduction was developed for determination of benzo[a]pyrene (bap) in bread by gas chromatography-mass spectrometry single quadrupole selected ion monitoring (gc/ms-sq-sim). sample preparation includes extraction of bap into acetone followed by cleanup with dispersive solid phase extraction. the use of spiked samples for constructing the calibration curve substantially reduced adverse matrix-related effects. the average recovery of bap at 6 concentration levels was in range of 95–120%. the method was proved to be reproducible with relative standard deviation less than 14.5% for all of the concentration levels. the limit of detection and limit of quantification were 0.3 ng/g and 0.5 ng/g, respectively. correlation coefficient of 0.997 was obtained for spiked calibration standard over the concentration range of 0.5-20 ng/g. to the best of our knowledge, this is the first time that a quechers method is used for analysis of bap in breads. the developed method was applied for determination of bap in 29 traditional (sangak) and industrial (senan) bread samples collected from tehran in 2014. these results showed that two sangak samples were contaminated with bap. therefore, a comprehensive survey for monitoring of bap in sangak bread samples seems to be needed. this is the first report concerning contamination of bread samples with bap in iran.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Validation of an Analytical Method for Determination of Benzo[a]pyrene Bread using QuEChERS Method by GC-MS

A fast and simple modified QuEChERS (quick, easy, cheap, rugged and safe) extraction method based on spiked calibration curves and direct sample introduction was developed for determination of benzo[a]pyrene (BaP) in bread by gas chromatography-mass spectrometry single quadrupole selected ion monitoring (GC/MS-SQ-SIM). Sample preparation includes extraction of BaP into acetone followed by clean...

full text

Validation of an Analytical Method for Determination of Benzo[a]pyrene Bread using QuEChERS Method by GC-MS

A fast and simple modified QuEChERS (quick, easy, cheap, rugged and safe) extraction method based on spiked calibration curves and direct sample introduction was developed for determination of benzo[a]pyrene (BaP) in bread by gas chromatography-mass spectrometry single quadrupole selected ion monitoring (GC/MS-SQ-SIM). Sample preparation includes extraction of BaP into acetone followed by clean...

full text

Validation of an Analytical Method for Determination of Benzo[a]pyrene Bread using QuEChERS Method by GC-MS

A fast and simple modified QuEChERS (quick, easy, cheap, rugged and safe) extraction method based on spiked calibration curves and direct sample introduction was developed for determination of Benzo [a] pyrene (BaP) in bread by gas chromatography-mass spectrometry single quadrupole selected ion monitoring (GC/MS-SQ-SIM). Sample preparation includes: extraction of BaP into acetone followed by cl...

full text

Validation of an Analytical Method for Simultaneous Determination of 18 Persistent Organic Pollutants in Trout Using LLE Extraction and GC-MS/MS

Persistent organic pollutants, (POPs), are vast distributed compounds in environment which are recognized as one of the global pollution problems. These groups of materials being dangerous due to their high stability are accumulated in animal tissues and occurring in the food chain. One of the major paths through which persistent organic combinations access to human body is consuming polluted f...

full text

Validation of an Analytical Method for Simultaneous Determination of 18 Persistent Organic Pollutants in Trout Using LLE Extraction and GC-MS/MS

Persistent organic pollutants, (POPs), are vast distributed compounds in environment which are recognized as one of the global pollution problems. These groups of materials being dangerous due to their high stability are accumulated in animal tissues and occurring in the food chain. One of the major paths through which persistent organic combinations access to human body is consuming polluted f...

full text

Validation of an Analytical Method for Determination of 13 priority polycyclic aromatic hydrocarbons in mineral water using dispersive liquid-liquid microextraction and GC-MS

Dispersive liquid liquid microextraction (DLLME) combined with gas chromatography–mass spectrometry (GC–MS) was used for the extraction and determination of 13 polycyclic aromatic hydrocarbons (PAHs) in mineral water samples. In this procedure, the suitable combination of extraction solvent (500 µL chloroform) and disperser solvent (1000 µL acetone) were quickly injected into the water sample (...

full text

My Resources

Save resource for easier access later


Journal title:
the iranian journal of pharmaceutical research

جلد ۱۵، شماره ۲، صفحات ۴۶۵-۴۷۴

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023